Microplate readers and controlling software enable walk away data analysis. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. VMax . Step 4: Incubate at room temperature for 1 hour, protected from light. Published Dec 16, 2020 | Updated Dec 16, 2020. 10,700. Your applications, your modes, your choice. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 9. This process should take about 3 - 5 minutes. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?Sunnyvale, California -- October 5, 2017. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. Select Update Driver. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. When the user applies a set temperature of 37°C, the top is heated to 37. Monday to Friday 8am - 5pm GMT. 5: Schematic of the back panel of a reader. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is. The Synergy H4 is a convenient benchtop microplate reader that offers robust. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. FlexStation® 3 Multi-Mode Microplate Reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. Step 2. The SpectraMax ABS. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The instrument supports the following read modes:. The sample is typically placed in a cuvette through which light is sent horizontally. Temperature control and shaking come standard. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 50/50. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Weight. Fluorescence Microplate Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. Instrument settings can be saved as a protocol file. It then uses the pre-defined settings for the specified instrument to build a protocol. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. After reading the ELISA plate on both readers, each standard curve was nearly identical. Keywords: spectramax ABS microplate readers, spectramax ABS plus microplate readers, spectramax ABS readers, ABS plus microplate readers, ABS. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. Learn moreMolecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. We would like to show you a description here but the site won’t allow us. ** Some compatible filter sets are available for purchase from Life. Download PDF. Tristar 3 Multimode Microplate Reader. TECHNICAL NOTE. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. In . Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. PURPOSE. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. Microplate Reader Shelf. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SoftMax Pro GxP Software. SoftMax® Pro 7. ~ Bio-Tek ELx808 Microplate Reader $2,795. Molecular Devices products are warranted to meet the stated specifications. The SpectraTest® ABS1 Absorbance Validation Plate tests optical performance using NIST-traceable standards. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. SoftMax® Pro 7. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Plate reader settings used for data acquisition of bacterial growth curve. Turn the reader on and secure the USB cable. BMG Labtech - THERMOstar. The adapter is required for optimum performa nce with standard 96-well and 384-. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Fill a clean plate with water. Fig 2: Enzymatic response of synthetic compounds. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. They feature options and modules to address your specific research including objectives, filters, imaging modes, and environmental. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 7°C (gradient heating design). We therefore gathered salient. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Molecular Devices Software. What are the physical and environmental operating specifications for SpectraMax UV/Vis and Gemini plate readers? (With extra info on backup power UPS choices). The SpectraMax ABS Plus reader utilizes Molecular . degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. SpectraMax® Microplate Reader Suite of Products Knowledge Base. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Instrument settings can be saved as a protocol file. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). PathCheck sensor corrects optical pathlength & pipetting variation. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. The advantage of using labeled probes is detection specificity. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 7°C and the bottom to 36. Search. 0. #SpectraMax L Config) Methods. To interrupt the kinetic measurement, the. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. This offer includes SpectraMax i3x, SpectraMax. Wait 20 seconds. Single-mode Microplate Reader. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12Plate reader instructions Computer. The instrument settings were identical to those used in Example 1 above. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. It this case, the EMax Plus Microplate Reader was the default. 9000-0756. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Firmly grasp the snap connector beside the snap, then push the snap down. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. 561. Also refer to the Multimode Reader Comparison Chart Features. Timothy Bolus, MPM, MBA, Compliance Program Manager. 340 PC384. 1. Devices’ StakMax® Microplate Stacker in a matter of minutes and . were started immediately with a Molecular Devices FlexStation Plate Reader. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Visible absorbance -. EUROPE. 944. Turn on Plate Reader and wait for it to complete its start-up routine. One plate carrier adapter is provided with the instrument. 2. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. 8 foot) 4400-0002 Power cord, 1 meter (3. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535. Explore our fully-integrated,. For nearly 40 years, we have partnered with scientists to help them achieve landmark discoveries. 0. FlexStation® 3 Multi-Mode Microplate Reader. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Instrument settings can be saved as a protocol file. Luminescence is the emission of light by a substance as a result of a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence). The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. These robust readers have been placed in labs from. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Load up the plates that you need to run and walk away. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Versa Max. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Page 1 of 8 . S. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. #6590-0118) Filter 665/10 (Molecular Devices cat. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 0) OD (0. Open the top cover of the instrument. The adapter is required for optimum performance with standard 96-well and 384-. For biochemical assays, the washer removes unbound material and unreacted reagents. 5 µL of each sample was pipetted into a 96-well. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. 1). SpectraMax Paradigm Multi-Mode Safety Guide From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SAN JOSE, Calif. you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices Supplier: Molecular Devices Enables data acquisition in endpoint, kinetic, and spectral scan modes at. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofRead plate standard and mix. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. #Flex3) FlexStation 3 8-channel Pipettor Head (Molecular Devices cat. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. ®This document is provided to customers who have purchased Molecular Devices, Inc. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. Superior optical system includes a xenon flash lamp and features. V. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Versa. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. to that of dedicated single-mode readers. Eliminate time-dependent substrate addition steps. SoftMax Pro Software. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. An end-to-end solution for high-content imaging and analysis. The SpectraTest® ABS1 Absorbance Validation Plate**4 °C above ambient to 45 °C. To update the driver. adipocytes: insulin dose-response. This multi plate reader is excellent to work with for checking the U. For more sample throughput, both readers can be easily. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. The temperature in the plate chamber is ambient and isothermal. User selects appropriate wells for samples and control using plate configuration tool. in steps of 0. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Plate layout configuration. Select Start>Control panel>Device Manager. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 315 can't find zero order. Create custom fluorescence assays without having to purchase expensive filter sets. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. plate readers. SoftMax Pro Software. The adapter is required for optimum performance with standard 96-well and 384-. In . San Jose, California, June 5, 2018. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. Microplate Readers. Recertification service. The procedures for these tests are outlined in the instrument user manual. SpectraMax® Microplate Readers and SoftMax® Pro Software. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 190. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. This document is copyright protected and any reproduction of this document, in whole or. On the back, in the right hand top corner, there. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for . 6% for the on-board optics (monochromators). This can increase throughput and efficiency, thus saving time and space in the lab. 150 μL of MTT solvent was then added to each well to solubilize the formazan product, and the plate was placed on a shaker for 15 minutes. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. 0A Sunnyvale, CA Made in USAFor SpectraMax ABS Plus reader, r 2 = 0. Step 1: Open the SoftMax Pro IMAP FP protocol. Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. Flexibility to read a variety of plate formats. Published Dec 16, 2020 | Updated Dec 16, 2020. Flexibility to read a variety of plate formats. Molecular Devices’ AquaMax ® 4000 Microplate Washer aspirates. These plate readers in the list below, are no longer included with the new SoftMax Pro 6 reader menu. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 314 low lamp level warning. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Unparalleled performance on a personalized platform. PURPOSE. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. The primary mode is fluorescence. 0 × 107) RLU 0. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Luminescence reading is a secondary mode of the Gemini instruments. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. Combining multiple microplate technologies and detection modes into a single, more versatile unit may be an ideal choice especially if you have. The Spectratest® Abs1 plate, which is used for validationof Molecular Devices monochromator based readers, cannot be used to validate the Emax/Vmax readers. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 0200-6263. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Turn on Plate Reader and wait for it to complete its start-up routine. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Molecular Devices' microplate readers. Data analysis. One plate carrier adapter is provided with the instrument. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Uncontrolled Copy for Training and Reference Purposes Only . #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. Shop now. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. With SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. DOCUMENTATION. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. Cell line development teams will realize savings on time and resources,SpectraMax i3x. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. SpectraTest Multi-Mode Validation Plate User Guide SpectraTest Validation Plates and SoftMax Pro Software reader validation protocols. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. • Analyze data in a single plate, across multiple plates, or between different groups. Supplier: Molecular Devices The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Molecular Devices training courses and tutorials. 9. 1 up to 3600 sec. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Citations on Google Scholar. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. FLIPR Calcium 4 Assay Kit. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. Also refer to the Multimode Reader Comparison Chart Features. Reader Documentation Version No. absorbance plate readers. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Contact our support team with your questions regarding Molecular Devices products and applications. Sale price $3,495. Vmax® plate reader specifications. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Use a Phillips screwdriver to remove the screws, and then remove the plate from the instrument. Learn more about how absorbance is measured, and some key applications that utilize absorbance. Step 1: Capture antibody binds to ELISA plate wells. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax readers support page; Analyst and FlexStation reader support page; Plate handlers and washers support page; We are continuously adding material to the knowledge base. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Lab automation for high-throughput plate-based assays. Unplug the power cable from the back of the instrument. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of. 2. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Fluorescence Microplate Reader Comparison Chart. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Specifications subject to change without notice. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Extended linear dynamic range. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Page 1 of 8 . Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Multi-mode Microplate Readers. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. A. Supersedes 1. The SpectraMax 190 reader can be integrated with Molecular . 96 deep well plate: packed in sleeves of 6 , 24 per case. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Supplier: Molecular Devices. . The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. FlexStation™ II Systems provide integrated fluid transfer capability from a source platePlate Reader SpectraMax iD5 multi-mode system Plate Washer AquaMax 4000 Microplate Washer Robot PreciseFlex 400 Sample Handler Plate Storage Plate Hotels – 40 microtiter plates Controlling Software Genera Control Software Incubation LiCONiC STX44 Automated CO 2 Incubator Liquid Handler Microlab STAR Automated Liquid Handling The. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Clone. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. The adapter is required for optimum performa nce with standard 96-well and 384-. #X7060. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. S. Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor Jinfang Liao and Evelyn McGown Molecular Devices Corporation, 1311 Orleans Dr. 1 up to 3600 sec. Note that this procedure is only for the SpectraMax L model. a single plate read using any standard cuvette, or 96- or 384-well microplate. Devices Absorbance Microplate Readers. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Installation Qualification (IQ)the iD5 reader’s filter system. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. EMax. In an analogous manner, the same sample names were assigned to a CuvetteSet section. No accessories are needed and SoftMax® Pro Software. Molecular Devices Software. and fluorescence microplate readers—. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Cell Cytotoxicity assay procedureMolecular Devices. The cuvette must be in place when you read the plate. SoftMax Pro GxP Software. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The whole plate can be detect in a single run with ease and flexible. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Eight filter options provide simplified assay setup.